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ACROBiosystems ACROBiosystems Human SIRP alpha / CD172a Protein, His Tag (HPLC-verified)

Supplier:  ACROBiosystems SIAH52251MG

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Recombinant Protein;1MG;Human SIRP alpha, His Tag (HPLC-verified) (SIA-H5225) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL (Routinely tested).;FACS assay shows that Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL (Routinely tested).;FACS analysis shows that the binding of Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) to Jurkat expressing C

Catalog No. 50-195-6739


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