ACROBiosystems Recombinant Protein;Biotinylated Human SIRP alpha / CD172a Protein, Fc Tag, Avi Tag (Avitag™);HEK293;200UG;CDA,SIRP alpha,SHPS1,SIRPA,CD172A,BIT,SHPS-1,MFR,MYD-1,MYD1,PTPNS1,SIRPalpha,p84,SIRP-alpha,CD172

Supplier:  ACROBiosystems CDAH82F2200UG

Recombinant Protein;Biotinylated Human SIRP alpha / CD172a Protein, Fc Tag, Avi Tag (Avitag™);200UG;Biotinylated Human SIRP alpha, Fc Tag (CDA-H82F2) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 65.8 kDa. The protein migrates as 75-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alpha, Fc Tag (Cat. No. CDA-H82F2) with a linear range of 5-205 ng/mL (QC tested).;FACS assay shows that recombinant Biotinylated Human SIRP alpha, Fc Tag, Avi Tag (Cat. No. CDA-H82F2) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml (Routinely tested).;FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc Tag, Avi Tag (Cat.

Catalog No. 50-165-8393