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Enzo Life Sciences FLUOR DE LYS SIRT2 deacetylase substrate (0.5µmol)
Supplier: Enzo Life Sciences BMLKI1790005
FLUOR DE LYS-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. FLUOR DE LYS-SIRT2 deacetylase substrate is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with FLUOR DE LYS Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate). Purity: ≥95% (HPLC). Formulation: Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer. Long Term Storage: -80°C.
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