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Enzo Life Sciences FLUOR DE LYS SIRT2 fluorometric drug discovery assay kit (1x96wells)
Supplier: Enzo Life Sciences BMLAK5560001
A FLUOR DE LYS fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The FLUOR DE LYS SIRT2 assay is based on the FLUOR DE LYSSubstrate and FLUOR DE LYS Developer combination. The assay procedure has two steps. First, the FLUOR DE LYS SIRT2 Substrate, which comprises a unique peptide based on amino acids 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), is incubated with SIRT2. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYSDeveloper II produces a fluorophore. Alternative name: Sirtuin 2 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
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