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Enzo Life Sciences FLUOR DE LYS SIRT5 fluorometric drug discovery assay kit (100 tests)
Supplier: Enzo Life Sciences BMLAK5130001
The SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS-Succinyl Substrate/Developer combination. The FLUOR DE LYS-Succinyl Substrate, which comprises a single lysine residue, Nε-succinylated on its side-chain, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
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