Invitrogen™ Human SMAD1 (Phospho) [pS463/pS465] InstantOne™ ELISA Kit

Catalog No.	Quantity
50-112-9725	96 Tests

Catalog No. 50-112-9725 Supplier Invitrogen™ Supplier No. 858618211

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD1 (Ser463/465) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. SMAD1 is a member of a family of transcription factors involved in mediating signaling by growth and differentiation factors such as transforming growth factor beta (TGF-b) and bone morphogenic protein (BMP).

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Specifications

• Accession Number: Q15797
• Content And Storage: • User Manual
  • Phospho-SMAD1 (Ser463/465) Capture Antibody Reagent, 3 mL
  • Phospho-SMAD1 (Ser463/465) Detection Antibody Reagent, 3 mL
  • InstantOne Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AI528653; BSP1; BSP-1; dwarfin-A; Dwf-A; DWFC; hSMAD1; JV41; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Madh1; MADH5; MADR1; Mad-related protein 1; Mlp1; mMad1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; mothers-against-DPP-related 1; Mothers-against-DPP-related-1; MusMLP; OTTHUMP00000220456; SMAD; Smad 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; Smad1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1AI528653; BSP1; BSP-1; dwarfin-A; Dwf-A; DWFC; hSMAD1; JV41; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Madh1; MADH5; MADR1; Mad-related protein 1; Mlp1; mMad1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; mothers-against-DPP-related 1; Mothers-against-DPP-related-1; MusMLP; OTTHUMP00000220456; SMAD; Smad 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; Smad1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
• Gene ID (Entrez): 4086
• Gene Symbol: SMAD1
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Synonym: Mothers against decapentaplegic homolog 1
• Target: SMAD1
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov