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Innovative Research Inc Snake Venom Phosphodiesterase I Purified lyophilized 100un
Supplier: Innovative Research Inc IDBRPDEILY100UN
Snake Venom Phosphodiesterase I Purified lyophilized from Innovative Research is prepared by process of Williams, Sung and Laskowski, JBC, 236, 1130 (1961) and treated to inactivate contaminating 5’-nucleotidase activity according to Sulkowski and Laskowski, Biochim. Biophys. Acta, 240, 443 (1961). This is a lyophilized in vials with a concentration of 20 Units/mg dry weight.
This product is useful successively hydrolyzing 5’-mononucleotides from 3’-OH-terminated riboand deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. It is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids and completely inhibited by 5 mM EDTA. ATP, ADP and AMP are partial inhibitors.
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