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New England Biolabs, Inc. SOC Outgrowth Medium – 4 x 25 ml medium
Supplier: New England Biolabs, Inc. B9020S
Add SOC to freshly transformed E. coli cells and incubate for up to 1 hour before plating on selective media. 1X SOC Outgrowth Medium: 2% Vegetable Peptone 0.5% Yeast Extract 10 mM NaCl 2.5 mM KCl 10 mM MgCl2 10 mM MgSO4 20 mM Glucose.
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