Edvotek™ Southern Blot Analysis For DNA Fingerprinting

Description

Includes

instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™

Requires

• Horizontal gel electrophoresis apparatus
• DC power supply
• Waterbath (65°C)
• Visualization system (white light)
• Staining tray and net
• NaCl, NaOH, concentrated HCl
• 250mL beakers or flasks
• 10mL glass graduated cylinder
• 100mL graduated cylinder
• Distilled or deionized water
• Microwave, hot plate, or Bunsen burner
• Small plastic box or tray forceps

Advanced-level experiment for DNA fingerprinting.

This experiment introduces your students to Southern blotting as a tool for 'DNA Fingerprinting' in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Southern blotting
• Includes: Instructions, DNA samples for electrophoresis, Practice gel loading solution, UltraSpec-Agarose™, Electrophoresis buffer, Pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™
• Requires: Electrophoresis apparatus, Power supply, 65°C Water bath, DNA visualization system, Staining net and tray, Automatic micropipettes, Lab glassware, Microwave or hot plate, Distilled water, NaCl, NaOH, Concentrated HCl, Plastic wrap, Forceps
• Time Range: Electrophoresis: 45 minutes; Blot: overnight; Stain/Destain: 10 minutes
• Class Size: 5 Lab Groups