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Edvotek™ Southern Blot Analysis For DNA Fingerprinting
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Catalog No. S11207 Shop All EDVOTEK Products
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S11207 5 Lab Groups
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Catalog No. S11207 Supplier Edvotek™ Supplier No. 207
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Description

Includes

instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™

Requires

  • Horizontal gel electrophoresis apparatus
  • DC power supply
  • Waterbath (65°C)
  • Visualization system (white light)
  • Staining tray and net
  • NaCl, NaOH, concentrated HCl
  • 250mL beakers or flasks
  • 10mL glass graduated cylinder
  • 100mL graduated cylinder
  • Distilled or deionized water
  • Microwave, hot plate, or Bunsen burner
  • Small plastic box or tray forceps

Advanced-level experiment for DNA fingerprinting.

This experiment introduces your students to Southern blotting as a tool for 'DNA Fingerprinting' in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

Specifications

Grade 9 to Undergraduate
For Use With (Application) Southern blotting
Includes Instructions, DNA samples for electrophoresis, Practice gel loading solution, UltraSpec-Agarose™, Electrophoresis buffer, Pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™
Requires Electrophoresis apparatus, Power supply, 65°C Water bath, DNA visualization system, Staining net and tray, Automatic micropipettes, Lab glassware, Microwave or hot plate, Distilled water, NaCl, NaOH, Concentrated HCl, Plastic wrap, Forceps
Time Range Electrophoresis: 45 minutes; Blot: overnight; Stain/Destain: 10 minutes
Class Size 5 Lab Groups
Product Type Electrophoresis Kit
Product Title
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