SouthernBiotech™ Dapi-Fluoromount-G™ Clear Mounting Media
Manufacturer: SouthernBiotech™ 010020
Dapi-Fluoromount-G is a water-soluble, instant-blue nuclear probe fluorescing (455nm) compound for mounting fixed slides using a staining procedure having an aqueous final step. Mounting slides with Dapi-Fluoromount-G stains the cell nucleus and provides a semi-permanent seal for long-term storage of slide preparations.
- Prepare cytocentrifuge preps according to established procedures
- Fix and rehydrate preps
- Remove one slide at a time from aqueous buffer, add 1 drop of Dapi-Fluoromount-G directly to cell prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip
- Allow mounted preps to air dry for 5 minutes before examination
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