Recombinant full-length mouse SPARC produced in HEK293 mammalian cells and supplied as a C-terminal 6xHis-tagged, lyophilized 1 mg vial. The glycosylated protein is >95% pure by reducing SDS-PAGE, has low endotoxin, and is functionally validated for cell growth inhibition assays; formulation and storage recommendations are provided to ensure activity and stability for biochemical and cell-based studies.
- Expression in HEK293 cells preserves mammalian glycosylation.
- C-terminal 6xHis tag facilitates purification and detection.
- Greater than 95% purity by reducing SDS-PAGE.
- Low endotoxin level (<1 EU/μg) suitable for cell assays.
- Lyophilized formulation for extended shelf life.
- Includes UniProt accession and full amino acid sequence.
- Validated biological activity with reported ED50 and specific activity.
- Recommended storage at -20°C and use of carrier proteins for long-term stability.