ECM Bioscience Sphingosine Kinase 1 (Central region) Rabbit Polyclonal Antibody, ECM Biosciences

Supplier:  ECM Bioscience SP1621

This antibody was affinity purified using SK1 (Central Region) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein, as well as detects endogenous SK1 isoforms near 40 and 50 kDa in HeLa, MeWo, and PC3 cells. In mouse, the antibody detects SK1 in isolated cardiac myocytes, but not in SK1-null mice (Tao, R. et al. (2007) Cardiovas. Res. 74:56.). The immunogen was SK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. Accession #Q9NYA1. Applications = WB, E; Species Reactivity = Hu; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year. Other Names = SK1, SPHK1, SPK1

Catalog No. 50-172-0893