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ECM Bioscience Sphingosine Kinase 1 (Ser-225), phospho-specific Rabbit Polyclonal Antibody, ECM Biosciences
Supplier: ECM Bioscience SP1641
This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated SK1 (Ser-225) peptide before affinity purification using phospho-SK1 (Ser-225) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein phosphorylated with ERK2, as well as detects endogenous SK1 near 40 kDa in calyculin A treated HeLa, MeWo, and PC3 cells. The immunogen was Phospho-SK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse SK1 is at serine 224. Accession #Q9NYA1. Applications = WB, E; Species Reactivity = Hu; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year. Other Names = SK1, SPHK1, SPK1
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