MP Biomedicals™ SPINeasy™ DNA/RNA/Protein All-In-One Kit

Description

Fast and efficient isolation of DNA, RNA and protein from a single sample using spin column technology. Lysing Matrix A for thorough and complete tissue lysis with FastPrep instruments.

• SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica membrane spin columns to isolate DNA, RNA, and protein components from the same sample, without the use of toxic substances such as phenol and chloroform.
• The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds.
• DNA, RNA, and proteins are then sequentially purified from the same lysate.
• Briefly, DNA is absorbed onto the first spin column, while the flow-through is collected and used for RNA purification.
• The second spin column captures RNA and the flow-through from this step is used for protein extraction.
• DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted.
• In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed, and resuspended.
• Each molecular component is then immediately available for their respective downstream applications.

Specifications

• Content And Storage: Store at Room Temperature, Dry Environment
• Isolation Technology: Spin Column
• Sample Type: Blood, Cell Culture, Animal Tissue, Swabs
• Includes: 50 x Lysing Matrix A, 60 mL Lysis Buffer R, 30 mL Wash Buffer D, 12 mL Wash Buffer R, 10 mL Elution Buffer GD, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 80 mL Protein Precipitant, 100 x Column A with collection tube
• Quantity: 50 Preps

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.