Invitrogen™ Human SPINT2 (HAI-2) ELISA Kit

Description

ELISA

The Human SPINT2 (HAI-2) ELISA quantitates Hu SPINT2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SPINT2. Principle of the method The Human SPINT2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: O43291
• Assay Range: 3.28 to 800 pg/mL
• Assay Sensitivity: 3 pg/mL
• Calibration Range: 3 pg/mL to 800 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human HAI-2, less than 0.1% cross-reactivity with human HAI-1 and mouse HAI-2 B is observed
• Sufficient For: 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10653
• Gene Symbol: DIAR3,HAI-2,HAI2,Kop,PB
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Endocrinology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: DIAR3, HAI-2, HAI2, Kop, PB
• Target: HAI-2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins