Invitrogen™ Human STAT3 (Total/Phospho) InstantOne™ ELISA Kit

Catalog No.	Quantity
50-246-263	96 Tests

Catalog No. 50-246-263 Supplier Invitrogen™ Supplier No. 858610311

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human STAT3 in cell lysates. Detection of mouse and rat STAT3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

Specifications

• Accession Number: P40763
• Content And Storage: • User Manual
  • phospho-STAT3 (Tyr705) Capture Antibody Reagent, 1 x 1.5 mL
  • phospho-STAT3 (Tyr705) Detection Antibody Reagent, 1 x 1.5 mL
  • STAT3 (Total) Capture Antibody Reagent, 1 x 1.5 mL
  • STAT3 (Total) Detection Antibody Reagent, 1 x 1.5 mL
  • InstantOne Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110034C02Rik; acute phase response factor; acute-phase response factor; ADMIO; ADMIO1; APRF; AW109958; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer; activator of transcription; acute-phase response factor; signal transduction and activation of transcription 3; STAT; STAT3; STAT-3; stat3 protein; STAT3b1; STAT3b2; transcription factor; Unknown (protein for MGC:128731); wu:fc15d02; wu:fl59g06; z-Stat31110034C02Rik; acute phase response factor; acute-phase response factor; ADMIO; ADMIO1; APRF; AW109958; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer; activator of transcription; acute-phase response factor; signal transduction and activation of transcription 3; STAT; STAT3; STAT-3; stat3 protein; STAT3b1; STAT3b2; transcription factor; Unknown (protein for MGC:128731); wu:fc15d02; wu:fl59g06; z-Stat3; ADMIO; ADMIO1; APRF; HIES
• Gene ID (Entrez): 6774
• Gene Symbol: STAT3
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Protein Aliases: Acute-phase response factor
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Synonym: Signal Transducer and Activator of Transcription, APRF
• Target: STAT3
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min