Invitrogen™ Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit

Catalog No.	Quantity
50-246-264	96 Tests

Catalog No. 50-246-264 Supplier Invitrogen™ Supplier No. 858611211

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT5A/B in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

Specifications

• Accession Number: P42229, P51692
• Content And Storage: • User Manual
  • phospho-STAT5A/B (Tyr694/Tyr699) Capture Antibody Reagent, 3 mL
  • phospho-STAT5A/B (Tyr694/Tyr699) Detection Antibody Reagent, 3 mL
  • InstantOne Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AA959963; Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; mammary gland factor; transcription factor; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; STAT5; Stat5a; Stat5b; transcription factor STAT5BAA959963; Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; mammary gland factor; transcription factor; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; STAT5; Stat5a; Stat5b; transcription factor STAT5B; STAT5;MGF; STAT5
• Gene ID (Entrez): 6776, 6777
• Gene Symbol: STAT5A, STAT5B
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Synonym: Signal Transducer and Activator of Transcription 5
• Target: STAT5 alpha/beta
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov