Invitrogen™ Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit

Catalog No.	Quantity
85-861-1311	96 Tests

Catalog No. 85-861-1311 Supplier Invitrogen™ Supplier No. 858611311

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human STAT5A/B (Tyr694/Tyr699) in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Specifications

• Accession Number: P42229
• Content And Storage: • User Manual
  • STAT5A/B (Total) Capture Antibody Reagent, 1 x 1.5 mL
  • STAT5A/B (Total) Detection Antibody Reagent, 1 x 1.5 mL
  • phospho-STAT5A/B (Tyr694/Tyr699) Capture Antibody Reagent, 1 x 1.5 mL
  • phospho-STAT5A/B (Tyr694/Tyr699) Detection Antibody Reagent, 1 x 1.5 mL
  • InstantOne ELISA Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AA959963; mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5AA959963; mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5; MGF; STAT5
• Gene ID (Entrez): 6776
• Gene Symbol: STAT5A
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Target: STAT5 alpha
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov