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Biotium Steady-Luc™ Firefly HTS Assay Kit, 120 Assays
Supplier: Biotium 300281
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a single-step, homogeneous, high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity. The kit has reagents sufficient for 120 assays in a 96-well format.
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