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Biotium Steady-Luc™ Firefly HTS Assay Kit, Trial Size, 40 Assays
Supplier: Biotium 30028T
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a single-step, homogeneous, high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity. The kit is a trial size version and has reagents sufficient for 40 assays in a 96-well format.
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