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MilliporeSigma™ STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Catalog No. SCR530
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Quantity:
1 Kit

Description

Contains high titer polycistronic lentivirus and Polybrene transfection reagent that have been validated for generation of mouse and human induced pluripotent stem cells. MilliporeSigma™; STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences.

  • Use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations
  • EF1α-STEMCCA (OKSM) Lentivirus: (Part number CS204279) Three (3) vials containing 15μL of high titer lentivirus
  • Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50μL of 10mg/mL stock
TRUSTED_SUSTAINABILITY

Specifications

Product Type Lentivirus Reprogramming Kit
For Use With (Application) Stem Cell Culture
Quantity 1 Kit
Regulatory Status RUO
Content And Storage Lentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
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