MilliporeSigma™ STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Description

Contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs).

• STEMCCA vector is comprised of transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences
• Use of single polycistronic lentiviral vector significantly improves reprogramming efficiencies
• Reduces number of viral integrations

Components:

• EF1α-STEMCCA (OKSM) Lentivirus: (Part number CS204279) One (1) vial containing 15μL of high titer lentivirus
• Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50μL of 10mg/mL stock

Specifications

• Product Type: Lentivirus Reprogramming Kit
• Quantity: 1 Kit
• Regulatory Status: RUO
• Content And Storage: Stable for 3 months from date of receipt when stored at -20°C or -80°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C.