MilliporeSigma™ STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Description

Contains high titer polycistronic lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells.

• Components:
• EF1α-STEMCCA-LoxP (OKSM) Lentivirus: (Part number CS204439) One (1) vial containing 15μL of high titer lentivirus
• Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50μL of 10mg/mL stock

Note: Provided in two formats, dependent on whether working with a human or mouse system. Human reprogramming experiments require a higher multiplicity of infection (MOI) than mouse experiments. Therefore, the larger, three-vial kits are required for human reprogramming experiments. If you are reprogramming mouse embryo fibroblast cells, you have the option to use either the one- or three-vial kit, depending on how many wells you want to infect. Reprogramming other rodent somatic cells may require more than one vial of lentivirus to perform at optimal MOI.

Specifications

• Product Type: Lentivirus Reprogramming Kit
• For Use With (Application): Stem Cell Culture
• Quantity: 1 Kit
• Regulatory Status: RUO
• Content And Storage: Lentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.