Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Cell Target Type Cell Culture Media
  6. Stem Cell Culture Media
  7. A1000701

Gibco™ StemPro™ hESC SFM

Catalog No. A1000701
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A1000701 1 Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A1000701 Supplier Gibco™ Supplier No. A1000701
Only null left
Add to Cart
Add to Cart

Description

StemPro hESC SFM is a serum- and feeder-free medium specially formulated to support the growth and expansion of pluripotent stem cells (hESCs and iPSCs). It has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities while maintaining differentiation potential.

StemPro hESC SFM is a serum- and feeder-free medium specially formulated to support the growth and expansion of pluripotent stem cells (hESCs and iPSCs). It has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities while maintaining differentiation potential.

StemPro hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines, including BG01, BG02, BG03, H1, H9, and BG01V. In addition, it has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of hESCs to differentiate into all three germ line lineages.

StemPro hESC SFM components

  • StemPro hESC Supplement
  • DMEM/F-12 with GlutaMAX medium
  • Bovine serum albumin 25% (BSA)

Additional components required for complete medium (need to be purchased separately)

  • FGF BASIC (full length) REC HU (Cat. no. PHG0261)
  • 2-Mercaptoethanol (Cat. no. 21985-023)

Applications

Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Embryonic Stem Cells (ESCs)
Classification Serum-free
Form Liquid
Product Type Stem Cell Media
Serum Level Serum-free
With Additives GlutaMAX, Phenol Red
Manufacturing Quality cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product Line StemPro
Quantity 1 Kit
Shipping Condition Supplement - dry ice, Basal - ambient, BSA - ambient
Species Human
Content And Storage • StemPro™ hESC Supplement, store at -5 to -20°C and protect from light
• DMEM/F-12 with GlutaMAX medium, store at 2 to 8°C and protect from light
• Bovine serum albumin (BSA) 25%, store at 2 to 8°C and protect from light
Culture Type Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
Show More Show Less

Frequently Asked Questions (FAQs)

How do I prepare the StemPRO hESC SFM complete medium?

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.
Can StemPRO hESC SFM be used to culture mouse ESCs?

No, we don't recommend StemPro hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut DMEM and KnockOut SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).

Can StemPRO hESC SFM be used for new cell line derivations?

Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.

What cell lines has StemPRO hESC SFM been tested with?

StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.

Does StemPRO hESC SFM require the use of a basement membrane matrix?

Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.

What additional products do I need to make a complete StemPRO hESC SFM?

In addition to the StemPRO hESC SFM kit, you will also need the following to make a complete medium: full length recombinant human FGF (catalog # PHG0261) and 2-Mercaptoethanol (catalog # 21985-023).
What are the components of the StemPro hESC SFM kit?

The StemPro hESC SFM kit (Cat. No. A1000701) contains: - 500 mL DMEM/F-12 with GlutaMax (Cat. No. 10565-018)
- 10 mL StemPro hESC SFM Supplement 50X (Cat. No. A10006-01)
- 40 mL Bovine Serum Albumin 25% (BSA Solution) (Cat. No. A10008-01)
Note that the StemPRO hESC Supplement and BSA solution cannot be ordered individually.
How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).
How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).
What are ES cells?

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.

Has Gibco Geltrex Matrix been tested with StemPRO hESC serum-free Medium?

Yes, these products were designed to work together.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.