Invitrogen™ Human STK3 (MST2), GST Tag Recombinant Protein

Catalog No.	Quantity
PV4806	1 mg
PV4805	10 μg
PR8881A	100 μg

Catalog No. PV4806 Supplier Invitrogen™ Supplier No. PV4806

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length.

STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

Specifications

• Accession Number: Q13188
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.02% Triton X-100, 150mM NaCl, 50% glycerol, 2mM DTT, 0.5mM EDTA and no preservative; pH 7.5
• Gene ID (Entrez): 6788
• Molecular Weight (g/mol): 85.5 kDa
• Name: Human STK3 (MST2), GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 0610042I06Rik; KB-1458E12.1; KRS1; Mammalian STE20-like protein kinase 2; mess1; MST; Mst2; MST-2; MST2/C; MST2/N; Mst3; protein kinase homolog; serine/threonine kinase 3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Serine/threonine-protein kinase Krs-1; Ste20; STE20-like kinase MST2; STK3; wu:fc19e11; zgc:55383
• Common Name: MST2
• Gene Symbol: STK3
• Kinase Family: STE20 Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: Storage Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, 0.02% Trition X-100, 0.5 mM EDTA, 2 mM DTT, 50% Glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Kinase Group: Ser⁄Thr Kinases: STE Group
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length
• Form: Liquid
• Research Category: Drug Development