Thermo Scientific™ Storage Tube Protection

Catalog No.	Product Type	Quantity
12-565-177	Tube Wrap	15/Pk., 300/Cs.
15-350-23	Vial Holder	100/Pk., 100/Cs.
12-565-184	Cryo Storage Box	350/Pk., 350/Cs.
12-565-185	Cryo Storage Box	200/Pk., 200/Cs.

Catalog No. 12-565-177 Supplier Thermo Scientific™ Supplier No. 343958

Description

Give your cryogenic storage tubes and vials extra protection, such as when shipping or storing in liquid or vapor phase nitrogen with these storage accessories.

Give your cryogenic storage tubes and vials extra protection when needed with Thermo Scientific™ Storage Tube Protection accessories. For example, safeguard your samples when storing in liquid or vapor phase nitrogen, or when shipping samples. These cryogenic storage accessories, such as tube wraps, sleeves and mini-boxes, have safe handling in mind.

• Heat shrink/pinch each end of the Nunc Cryoflex tubing around cryostorage tubes to prevent leakage of liquid nitrogen into the tubes during storage
• Transparent Nunc Cryosleeve tubing fits any Thermo Scientific Nunc or Nalgene™ cane and provides extra security during handling and submersion of tubes into the liquid phase without the tubes touching the liquid nitrogen
• Nunc Cryotube Mini-boxes are ideal for storing small quantities of samples and shipping with a friction fit lid and durable high-impact polystyrene construction to protect samples

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Specifications

• Description: Nunc CryoFlex Tube Wrap, 500 mm, 14 mm ID
• Diameter (Metric) Inner: 14 mm
• Quantity: 15/Pk., 300/Cs.
• Height (Metric): 500 mm
• Product Type: Tube Wrap

Frequently Asked Questions (FAQs)

Will the heat required to properly seal Nunc CryoFlex Tube Wrap adversely affect my cells?

Nunc Biobanking and Cell Culture Cryogenic Tubes are normally placed in a 4 degrees C ice bath during cell inoculation in the cryogenic tube. The heat required to shrink the polyethylene Nunc CryoFlex Tube Wrap will not raise the internal temperature enough to damage the cells. At this stage, cryoprotective agents are added.

What protocol should I follow for thawing cells, if my Nunc Biobanking and Cell Culture Cryogenic Tubes were previously immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap?

We recommend the following protocol for thawing cells immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap:
- Wear appropriate protective clothing including gloves, face guard, etc., during all transfer processes.
- The Nunc Biobanking and Cell Culture Cryogenic Tubes should be stored at least 24 hr in the gas phase of the liquid nitrogen freezer. This should allow trapped liquid nitrogen to evaporate and escape slowly, thereby reducing the possible pressure in the cryogenic tube.
- After at least 24 hr storage in the gas phase, the cryogenic tube may be put into a water bath and covered with a towel.
- After thawing, the contents of the cryogenic tube can be transferred.

Can I immerse Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid nitrogen (liquid phase) for storage?

Of the Nunc Biobanking and Cell Culture Cryogenic Tubes, we recommend the internally threaded design, incorporating a silicon gasket, for liquid nitrogen immersion. At ultra low temperatures, any sealing material becomes inflexible. A theoretical risk of leakage is present when any mechanical closure is used. To facilitate leakproof storage, mechanically closed containers must be hermetically sealed in a protective envelope.

The Nunc cryo products includes Nunc CryoFlex Tube Wrap, a heat sealable polyethylene tubing designed for cryogenic tube encasement. The use of cryogenic tubes for freezing in liquid nitrogen (liquid phase) storage without CryoFlex tubing poses a safety hazard.
WARNING: Do not use Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid phase of liquid nitrogen unless correctly sealed in Nunc CryoFlex Tube Wrap (Cat. No. 343958). Improper use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up, resulting in possible explosion or biohazard release. Use appropriate safety procedures as outlined in the Nalge Nunc International Cryopreservation Manual when handling and disposing of the vials.