Invitrogen™ Streptavidin, Alexa Fluor™ 405 conjugate

Catalog No.	Quantity
S32351	1 mg

Catalog No. S32351 Supplier Invitrogen™ Supplier No. S32351

Description

Streptavidin, Alexa Fluor™ 405 conjugate

Our streptavidin, Alexa Fluor 405 conjugate is a bright, photostable probe with excitation/emission maxima of 402 and 421 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

Specifications

• Conjugate: Alexa Fluor 405
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
• Quantity: 1 mg
• Content And Storage: Contains 1 vial of streptavidin Alexa Fluor™ 405 conjugate.
  
  Store in freezer (-5 to -30°C) and protect from light.
• Excitation/Emission: 402/421
• Flow Cytometer Laser Lines: 405
• Shipping Condition: Room Temperature
• Form: Solid
• Product Line: Alexa Fluor
• Product Type: Streptavidin Conjugate (Fluorescent)
• Label or Dye: Alexa Fluor 405

Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

For Research Use Only. Not for use in diagnostic procedures.