Molecular Probes Streptavidin, Alexa Fluor 488 Conjugate
Streptavidin, Alexa Fluor 488 conjugate
Manufacturer: Molecular Probes S32354
DescriptionAlexa Fluor 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor 488 Streptavidin Conjugates:
Properties of Alexa Fluor Dyes
Alexa Fluor dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
|Store in refrigerator (2–8°C) and protect from light.|
|Alexa Fluor™ 488|
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For Research Use Only. Not for use in diagnostic procedures.