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Invitrogen™ Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)

Catalog No. 501121178
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50-112-1178 100 μL
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Catalog No. 50-112-1178 Supplier Invitrogen™ Supplier No. S21008
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Description

Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)

The streptavidin, Alexa Fluor™ 750-allophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at ∼650/779 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following excitation at 633 nm or 647 nm.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.
TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 mg/mL
Conjugate APC-Alexa Fluor 750
Detection Method Fluorescence
For Use With (Equipment) Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Quantity 100 μL
Content And Storage Contains 1 vial of streptavidin APC-Alexa Fluor™ 750 conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.
Excitation/Emission 633 or 635/775
Flow Cytometer Laser Lines 633 or 635
Shipping Condition Wet Ice
Form Solution
Product Line Alexa Fluor
Product Type Streptavidin Conjugate (Fluorescent)
Label or Dye APC-Alexa Fluor 750
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Frequently Asked Questions (FAQs)

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.
How should I store my allophycocyanin (APC)-conjugated antibody?

Allophycocyanin-conjugated antibodies must be stored at 2-8 degrees C. Antibodies conjugated to phycobiliproteins (APC, R-PE) must never be frozen. Once frozen, even briefly, they are no longer usable.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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