Molecular Probes™ Streptavidin, Alexa Fluor™ 790 Conjugate

Catalog No.	Quantity
S11378	1 mg

Catalog No. S11378 Supplier Molecular Probes™ Supplier No. S11378

Description

Streptavidin, Alexa Fluor™ 790 Conjugate

Alexa Fluor™ 790 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 790 Streptavidin Conjugates:

Alexa Fluor™ 790 streptavidin conjugate has Ex/Em maxima of ∼(782/805)

Bright, photostable fluorescence

High solubility in aqueous solutions

Available in multiple colors

Ideal for western blots

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor™ Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates—Section 7.6 in the Molecular Probes Handbook

Specifications

• Conjugate: Alexa Fluor 790
• Quantity: 1 mg
• Content And Storage: Store at ≤-20°C
  
  Protect from light
  
  Desiccate
• Shipping Condition: Room Temperature
• Form: Solid
• Product Line: Alexa Fluor
• Product Type: Streptavidin Conjugate (fluorescent)
• Label or Dye: Alexa Fluor Dyes

Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Safety and Handling

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