Invitrogen™ Streptavidin, Pacific Blue™ conjugate

Catalog No.	Quantity
50-112-1543	1 mg

Catalog No. 50-112-1543 Supplier Invitrogen™ Supplier No. S11222

Description

Invitrogen™'s streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm.

Invitrogen™'s streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

Specifications

• Color: Yellow
• Conjugate: Pacific Blue
• Description: Pacific Blue™ Conjugate Streptavidin, 1mg, Yellow, Solid Form, 407nm Absorbency, Pacific Blue™ Label or Dye, 410/455nm Excitation/Emission, Used in Blot Analysis, Flow Cytometry, Histochemical Applications, Immunoassays, –5 to –30°C Storage Temperature
• Detection Method: Fluorescence
• Formulation: Solid
• pH: 8
• Quantity: 1 mg
• Regulatory Status: For research use only (RUO)
• Sample Volume: 1 mg
• Packaging: 1 Each
• Storage Buffer: 10mg/mL PBS
• Content And Storage: Contains 1 vial of streptavidin Pacific Blue™ conjugate.
  
  Store in freezer (-5 to -30°C) and protect from light.
• Excitation/Emission: 410/455 nm
• Flow Cytometer Laser Lines: 405
• Shipping Condition: Room Temperature
• Form: Solid
• Product Line: Pacific Blue
• For Use With (Application): Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
• Purity or Quality Grade: TLC
• Product Type: Streptavidin Conjugate
• Label or Dye: Pacific Blue

Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.