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Bioworld Blot Stripping Buffer (4X), 500 mL
Supplier: Bioworld 209600042
Product DescriptionBlot stripping buffer (4x) contains enough liquid concentrate to make 1L of 1X solution. It contains 250 mM Tris and 8% SDS. At 25°C, it has a pH value of 6.8.To use this product, dilute it 4 fold with deionized (DI) water and add 0.6% of β-Mercaptoethanol (beta ME). Wash the membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 minutes in PBS-tween20 or TBS-tween20.Product is an irritant hazard.
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