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Novus Biologicals™ Subcellular Protein Fractionation Kit
SDP

Catalog No. NBP247659 Shop All R&D Systems Products
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NBP247659 50 Assays
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Catalog No. NBP247659 Supplier Novus Biologicals™ Supplier No. NBP247659

Description

Designed to provide reproducible extraction of four subcellular protein fractions (cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample; the percentage of proteins in the different fractions are cell-dependent, usually in the range of approx.20-30% cytosol, approx.25-35% membrane (organelles), approx.15-25% Nuclei, approx.15-25% insoluble

In Western analysis, if a membrane protein appeares mainly in the nuclear fraction, the membrane extraction step may not be sufficient. First, make sure to add Membrane Extraction Buffer B into the extraction, which contain NP-40 detergent. If it still shows the same result, increase incubation time from 1 min to 5 min after adding MEB-B. NP-40 should extract membrane from most of cells.

Specifications

Includes Protein Fractionation Kit
Product Type Subcellular Fractions
Target Proteins Subcellular Protein Fractionation
Content And Storage Store at -20°C Avoid freeze-thaw cycles
For Use With (Application) 1-D or 2-D gel, enzyme activity assays, gel supershift assay, and Western blotting; Subcellular Protein Fractionation: The Cell Fractionation Kit can be used in 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Quantity 50 Assays
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