Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Description

Fractionate tissue sample-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins within 2 hours.

The Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours.

The complete Subcellular Protein Fractionation Kit for Tissue includes a unique 250μm tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization. The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination. Tissue extracts obtained with this subcellular fractionation kit are compatible with a variety of downstream applications, including Western blotting, protein quantitation, electrophoretic mobility shift, reporter-gene and enzyme-activity assays.

Highlights:

• Optimized formulations and protocols specific for fractionation of tissue

• Complete obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit

• Time efficient extract functional protein fractions without ultracentrifugation in approximately 2 hours

• Convenient unique tissue strainer allows rapid removal of tissue debris

• Compatible use extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme-activity assays

• Versatile extract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen

Recommended for:

Obtain cytoplasmic, soluble nuclear, chromatin-bound, membrane and cytoskeletal proteins from the same tissue sample; Profile disease-state versus normal tissues for proteins from the cytoplasm, nucleus, membrane and cytoskeleton; Study protein translocation in tissues

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Specifications

• Product Type: Subcellular Protein Fractionation Kit
• Content And Storage: Upon receipt store kit at -20°C or store individual components as indicated above. Kit is shipped with dry ice.
• Form: Liquid
• Buffer: Tissue Extraction Buffer, Lysis Buffer
• Reagent Type: Tissue Extraction Buffer, Detergent Solution, Cell Lysis Buffer, Subcellular Fractionation
• Sufficient For: 25 Samples
• Quantity: 1 Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov