Invitrogen™ Substance P Competitive ELISA Kit

Catalog No.	Quantity
EEL013	96 Tests

Catalog No. EEL013 Supplier Invitrogen™ Supplier No. EEL013

Description

ELISA

The multispecies SP (Substance P) ELISA quantitates SP in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure SP(Substance P) independent of species. A SP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to SP added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of SP in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Substance P (SP) is currently thought to be one of the most important neurotransmitters and neurotransmitters in the brain. It is an undecapeptide belonging to the tachykinin family, which consists of excitatory neuropeptides synthesised in neuronal and glial cells of the human central and peripheral nervous system. Substance P, Neurokinin A, Neuropeptide K, and Neuropeptide gamma are all generated by post-translation cleavage of the precursor Protachykinin-1. Substance P forms the major endogenous ligand for Neurokinin 1 Receptor. The pharmacology of Substance P has been associated with a number of neurological and psychiatric disorders, namely nociception, migraine, asthma, nausea, inflammatory bowel syndrome, urinary incontinence, anxiety and depression. It has also been linked to obesity.

Specifications

• Assay Range: 78.13-5000 pg/mL
• Assay Sensitivity: 46.88 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Tachykinin neuropeptide
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 4930528L02Rik; C-terminal-flanking peptide; Hs.2563; MGC26719; neurokinin 1; neurokinin 2; neurokinin A; neurokinin alpha; neuromedin L; Neuropeptide gamma; neuropeptide K; NK1; NK-1; NK2; Nka; Nkna; NPK; PPT; Ppt5fl; PPTA; PPT-A; PPTA3; precursor 1; preprotachykinin; preprotachykinin A; protachykinin; protachykinin-1; RAT TAC; RATPPTA3; SP; substance K; Substance P; TAC; Tac1; TAC2; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma); tachykinin 1; tachykinin 2; tachykinin precursor 1; tachykinin, precursor 1; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)4930528L02Rik; C-terminal-flanking peptide; Hs.2563; MGC26719; neurokinin 1; neurokinin 2; neurokinin A; neurokinin alpha; neuromedin L; Neuropeptide gamma; neuropeptide K; NK1; NK-1; NK2; Nka; Nkna; NPK; PPT; Ppt5fl; PPTA; PPT-A; PPTA3; precursor 1; preprotachykinin; preprotachykinin A; protachykinin; protachykinin-1; RAT TAC; RATPPTA3; SP; substance K; Substance P; TAC; Tac1; TAC2; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma); tachykinin 1; tachykinin 2; tachykinin precursor 1; tachykinin, precursor 1; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma);
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Substance P
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min