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Enzo Life Sciences FLUOR DE LYS-Succinyl Green (50µl)
Supplier: Enzo Life Sciences BMLKI5910050
The FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer (BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm). . Formulation: Solution in DMSO. Purity: ≥95% (HPLC). Long Term Storage: -80°C.
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