Apexbio Technology LLC Sulfo-NHS-Biotin Kit (90-180 kDa) 3samples

Supplier:  Apexbio Technology LLC K11513

Sulfo-NHS-Biotin labeling is widely utilized in biochemical research to covalently conjugate biotin molecules to free amino groups on protein molecules primarily lysine residues and N-terminal amines The reaction proceeds via activated ester intermediates forming stable amide bonds that facilitate subsequent detection or purification using streptavidin-biotin affinity interactions This labeling method is routinely applied to diverse protein samples particularly immunoglobulin G and other antibodies (molecular weight 90-180 kDa) enabling downstream applications such as immunoassays Western blotting immunoprecipitation and flow cytometry analyses Commonly the labeled proteins are purified rapidly by spin-based filtration to eliminate unreacted small molecules improving purity and yield

Catalog No. 50-000-63451