Thermo Scientific™ SureSTART™ 0.3 mL Glass Snap Top (U)HPLC and GC Vials with Fixed-Insert, Level 3

Catalog No.	Color	Quantity
03-452-366	Amber	5000/Pk.
03-452-353	Clear	100/Pk.
03-452-365	Clear	5000/Pk.
03-452-359	Clear	1000/Pk.
03-452-354	Amber	100/Pk.
03-452-360	Amber	1000/Pk.

Catalog No. 03-452-366 Supplier Thermo Scientific™ Supplier No. 6PRV1103FIVAPF

Description

For limited-volume LC-MS and GC-MS analyses that demand reproducible, high-sensitivity results, use SureSTART™ 300 μL Glass Snap Top Vial with Fixed-Insert (Level 3).

Thermo Scientific™ SureSTART™ 300 μL Glass Snap Top Vials with Fixed Insert (Level 3) are the premier choice for high-sensitivity LC-MS and GC-MS workflows when working with small sample volumes (<2 mL).

The fixed glass insert provides a total volume of 300 μL (0.3 mL) with a usable volume of 250 μL and a residual volume of ~4 μL. The vials deliver reproducible results and exceptional sample protection for demanding applications.

Made with high-purity clear or amber glass, these vials contain the lowest metal content, extractables, and leachables - critical for meeting US, EU, and JPN Pharmacopeia standards. Also ensures consistent sensitivity and selectivity across HRAM (high-resolution accurate mass) instruments including Thermo Scientific™ LC and GC Orbitrap™ MS systems. Combine with Thermo Scientific™ SureSTART™ 11 mm snap caps and septa, to ensure optimal performance.

Key Features

• 12 x 32 mm standard vial with fixed 300 μL insert (0.3 mL total volume)
• Provides 250;L usable volume and ~4 μL residual volume
• Available in clear and amber glass
• For a secure, leak free seal, pair with SureSTART™ 11 mm snap caps and septa
• Write-on patch for sample labeling and tracking

Performance Level 3 – for High-Sensitivity Applications

• Identified by three waves on the vial
• Meets US, EU, and JPN Pharmacopeia requirements
• Contain the lowest levels of extractable metals and leachables
• Ideal for GC, LC, and MS applications, including Thermo Scientific™ LC and GC Orbitrap™ MS systems and other HRAM instruments
• Supports workflows that demand consistent sensitivity and selectivity

Specification Certified

• Dimensionally verified for complete autosampler compatibility
• Inspected via automated optical and gauge systems to maintain strict tolerances
• Constructed from uniform, defect-free glass for consistent performance

Lot Traceable

• 100% traceable through the manufacturing process
• Certificate of Conformance available by lot number for audit documentation

Specifications

• Diameter (Metric) Opening: 4.5 mm
• Color: Amber
• Diameter (Metric) Outer: 12 mm
• Material: Glass
• Sterility: Non-sterile
• Disposable: Single-use
• Volume (Metric): 0.3 mL
• For Use With (Application): (U)HPLC, GC, MS
• Graduated: Yes
• Insert Type: Fixed Insert
• Material (Coating): No
• Packaging Type: 50 Packs of 100
• Size (Metric) Opening: 11 mm
• Volume (Metric) Residual: <4 μL
• Base Style: Outer Base Style: Flat Bottom Inner Base Style: Conical Insert
• Product Line: SureSTART Level 3
• Volume (Metric) Total: 0.30 mL
• Closure Type: Snap Cap
• Quantity: 5000/Pk.
• Closure Size: 11 mm
• Marking Spot: Yes
• Volume (Metric) Usable: 0.25 mL
• For Use With (Equipment): HPLC/GC Autosampler
• Autoclavable: Autoclavable
• Certifications/Compliance: Specification Certified
• Height (Metric): 32 mm
• Product Type: Snap

Frequently Asked Questions (FAQs)

What are SureSTART fixed insert vials?

During manufacture, the outer vial has a glass micro-insert precisely positioned inside and this is permanently fused to the base of the outer vial. This gives a 12 mm x 32 mm microvial with a capacity of 350 µL with a narrow reservoir with <10 µL capacity. A fixed insert vial removes the need to assemble a vial and insert prior to use.

What are SureSTART microvials?

Microvials are suitable for use when sample volume is limited or when you need to get maximum recovery from the vial. These vials allow small volumes to be sampled accurately by autosamplers with a recoverable volume of much less than 100 µL. High recovery, total recovery, and vials with fixed inserts are all classed as microvials.