Thermo Scientific™ SureSTART™ 2 mL GOLD-Grade Glass (U)HPLC and GC Vials, Level 3

Catalog No.	Product Type	Thread Style	Quantity
03-452-241	SureStop Screw Thread	9 mm SureStop Short Thread	100/Pk.
03-452-201	Crimp	11 mm	100/Pk.
03-452-245	SureStop Screw Thread	9 mm SureStop Short Thread	5000/Pk.
03-452-243	SureStop Screw Thread	9 mm SureStop Short Thread	1000/Pk.

Catalog No. 03-452-241 Supplier Thermo Scientific™ Supplier No. 6PSV91PG

Description

For high-performance LC-MS and GC-MS analyses of polar or pH-sensitive analytes, use SureSTART™ 2 mL GOLD-Grade Glass Screw and Crimp Top Vials (Level 3). SureStop™ technology is available on the screw top format

Thermo Scientific™ SureSTART™ 2 mL GOLD-Grade Glass Screw and Crimp Top Vials (Level 3) are the elite choice for high-sensitivity LC-MS, GC-MS, and UHPLC workflows where analytes such as polar compounds or those affected by pH changes demand maximum sample stability and ultra-low adsorption.

These vials are made from Thermo Scientific™ GOLD-Grade low-adsorption glass, offering:

• Highest glass purity available
• Lowest surface activity
• Minimal pH shift and ultra-low adsorption for trace-level analysis.

The screw-top version features Thermo Scientific™ SureStop™ technology, which reduces cap tilt, septum push-through, and evaporation – maintaining sample integrity throughout demanding analyses. To ensure optimal performance, pair with Thermo Scientific™ SureSTART™ Advanced Vial Closure System (AVCS™) 9 mm caps and septa.

The crimp-top version, combine with 11 mm Thermo Scientific™ SureSTART™ crimp caps and septa. This will provide a tight, solvent-resistant seal ideal for volatile samples and long-run LC/GC applications, ensuring maximum sample security under challenging conditions.

Key Features

• Constructed from ultra-pure, low-adsorption glass to reduce analyte interaction, minimizing pH shift for trace and sensitive compound analysis
• Offered in screw-top and crimp-top formats
• Screw top vials include SureStop™ technology for improved sealing consistency – pair screw top vials with SureSTART™ 9 mm AVCS™ screw caps and septa for a secure, leak-free seal
• Crimp top vials, when paired with 11 mm Thermo Scientific™ SureSTART™ crimp caps and septa, deliver tight, solvent-resistant seals for volatile samples and extended run times. Use Thermo Scientific™ manual or electronic crimpers and decrimpers to seal and remove your crimp caps
• Write-on patch for sample labeling and tracking

Performance Level 3 – GOLD-Grade for Ultra-Trace Applications

• Identified by three waves on the vial
• Meets US, EU, and JPN Pharmacopeia requirements
• Contain the lowest levels of extractable metals and leachables
• Ideal for GC, LC, and MS applications, including Thermo Scientific™ LC and GC Orbitrap™ MS systems and other HRAM instruments
• Supports workflows that demand consistent sensitivity and selectivity

Specification Certified

• Dimensionally verified for complete autosampler compatibility
• Inspected via automated optical and gauge systems to maintain strict tolerances
• Constructed from uniform, defect-free glass for consistent performance

Lot Traceable

• 100% traceable through the manufacturing process
• Certificate of Conformance available by lot number for audit documentation

Specifications

• Diameter (Metric) Opening: 6 mm
• Color: Clear
• Diameter (Metric) Outer: 12 mm
• Material: Glass
• Sterility: Non-sterile
• Disposable: Single-use
• Volume (Metric): 2 mL
• For Use With (Application): (U)HPLC, GC, MS
• Graduated: Yes
• Insert Type: None
• Material (Coating): No
• Packaging Type: 1 Pk. of 100
• Size (Metric) Opening: 9 mm
• Thread Style: 9 mm SureStop Short Thread
• Volume (Metric) Residual: <170 μL
• Base Style: Outer Base Style: Flat Bottom Inner Base Style: Flat Bottom
• Product Line: SureSTART Level 3
• Volume (Metric) Total: 2 mL
• Closure Type: Screw Cap
• Quantity: 100/Pk.
• Closure Size: 9 mm
• Marking Spot: Yes
• Volume (Metric) Usable: 1.5 mL
• For Use With (Equipment): HPLC/GC Autosampler
• Autoclavable: Autoclavable
• Certifications/Compliance: Specification Certified
• Height (Metric): 32 mm
• Product Type: SureStop Screw Thread

Frequently Asked Questions (FAQs)

Are SureSTOP vials compatible with standard autosamplers, or do I need special equipment?

SureSTOP vials are designed for compatibility with nearly every LC or GC autosampler. They are part of the SureSTART family, which is tested for autosampler compatibility and specification certified for physical dimensions.

How do SureSTOP vials help with evaporation control compared to generic vials?

Under stress conditions (e.g., 1.3 mL methanol at 40 °C for 72 h), SureSTOP vials with AVCS closures lose significantly less solvent compared with generic screw-top or even crimp vials. The “definite stop” means the cap is tightened correctly (neither under nor over-tightened) so septum compression is predictable, and cap-tilt or septum displacement (which lead to evaporation paths) are minimized.

In workflows where solvent loss or sample concentration change is critical (as for very low volumes or volatile analytes), SureSTOP vials offer measurable improvement.

What types of applications are SureSTOP vials well-suited for?

SureSTOP vials are well-suited for:
• GC, LC and MS workflows where volatile or semi-volatile solvents are used and evaporation or sample loss is a concern.
• High-sensitivity or trace-analysis workflows where variation in sealing can impact reproducibility.
• Labs where autosampler failures (due to cap/tilt/septum issues) are affecting throughput.

How are SureSTOP vials different from standard screw-top autosampler vials?

Key aspects of SureSTOP vials include:
• The “definite stop” design prevents over-tightening (which might deform the septum or the vial neck) and under-tightening (leading to leaks or evaporation).
• Achieve the best performance with caps that have Advanced Vial Closure System (AVCS) technology that levels and aligns the cap properly, reducing cap tilt and septum displacement.
• Demonstrated lower solvent loss (evaporation) in stressed tests compared with generic vial and closure combinations.

What problems do SureSTOP vials help solve?

The SureSTOP vials are 2 mL screw-thread (9 mm) vials that incorporate a patented “definite stop point” in the vial finish (alignment ring) so when you use them with AVCS (Advanced Vial Closure System) 9 mm screw caps, you cannot under- or over-tighten the closure. This feature helps solve problems such as inconsistent sealing, variable septum compression, vial/septa push through, sample evaporation, or autosampler failures due to poorly sealed vials.

What type of vial should I use for chromatographic/chromatogenic analysis of polar compounds?

For very polar compounds, including ionizable biomolecules and inorganic ions, the use of a plastic vial may be considered. Polypropylene vials are included in the SureSTART range as a Level 1 range of products.

Most polar analytes can be analyzed successfully in glass vials with few problems at normal analytical levels. Low concentrations may require investigation of deactivated vials, but in most cases the control of the sample pH is enough to maintain linearity.

Type 1 neutral borosilicate glass is suitable for neutral/acid samples with little change of pH caused by the glass surface.

When would I need inert vials for my chromatography samples?

For analysis of compounds which show interactions with untreated glass surfaces, it may be necessary to use a deactivated glass vial. The effect of adsorption is most apparent when dealing with very low concentrations of the sample. It is likely that this effect will only be identified during calibration. Analytical results will show poor reproducibility and linearity for active samples while non-adsorbing species show good results.

Inert glass products can be found in Performance level 3, and include silanized glass and GOLD-grade technology deactivated glass.

What are SureStop vials?

In addition to the features on standard 2 mL vials, SureStop vials also have a patented definite stop point (alignment ring) in the design of the vial finish. This prevents the vial from being an optimal seal, which eliminates under- and over-sealing by the cap/closure and user-to-user variance.

SureStop vials are designed for use with Advanced Vial Closure System (AVCS) screw caps. They improve transfer reliability and give a more secure liquid seal than standard screw vials. SureStop vials are available as a 2 mL as either clear or amber options and are highly recommended for use with GC and LC applications.