Invitrogen™ Human Syk, GST Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length, Recombinant, Cytoplasmic

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

Specifications

• Accession Number: P43405
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris HCl with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 6850
• Molecular Weight (g/mol): 100.1 kDa
• Name: Human Syk, GST Tag
• Quantity: 10 μg
• Gene Alias: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72Syk; p72-Syk; ptk72; SP1; spleen associated tyrosine kinase; spleen tyrosine kinase; SYK; syk signaling; Sykb; tyrosine-kinase syk; tyrosine-protein kinase SYK
• Common Name: Syk
• KinaseFamily: SYK Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate
• Tag Position: N-Terminal
• Target Entry: SYK
• Target Gene: spleen tyrosine kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length, Recombinant, Cytoplasmic
• Form: Liquid
• Protein Subtype: Cytoplasmic Tyrosine Kinases
• Research Category: Oncology, Signal Transduction