Invitrogen™ Human Syndecan 1 (SDC1) ELISA Kit

Description

ELISA

The Human Syndecan 1 (SDC1) ELISA quantitates Hu Syndecan 1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Syndecan 1. Principle of the method The Human Syndecan 1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

Specifications

• Accession Number: P18827
• Assay Range: 16.38 to 4000 pg/mL
• Assay Sensitivity: 14 pg/mL
• Calibration Range: 14 pg/mL to 4000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human Syndecan-1. Other species not determined.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6382
• Gene Symbol: CD138,SDC,SYND1,syndecan
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 15 μL, Serum 15 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: CD138, SDC, SYND1, syndecan
• Target: CD138 (Syndecan-1)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins