SYPRO Ruby protein gel stain is a ready-to-use ultrasensitive luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain designed especially for use in 2-D PAGE has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEf) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However unlike silver staining the SYPRO Ruby gel stainuses a simple staining protocol with no possibility of overstainingdelivers a linear quantitation range of over three orders of magnitudeshows less protein-to-protein variabilitystains glycoproteins lipoproteins calcium-binding proteins fibrillar proteins and other difficult-to-stain proteinswill not stain extraneous nucleic acidsdoes not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry