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New England Biolabs, Inc. T4 Polynucleotide Kinase – 2500 units
Supplier: New England Biolabs, Inc. M0201L
T4 PNK catalyzes the transfer and exchange of P from the gamma; position of ATP to the 5 -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3-phosphoryl groups from 3-phosphoryl polynucleotides, deoxynucleoside 3-monophosphates and deoxynucleoside 3-diphosphates.
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
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