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New England Biolabs, Inc. T4 RNA Ligase 2, truncated – 10000 units
Supplier: New England Biolabs, Inc. M0242L
T4 RNA Ligase 2' truncated (T4 Rnl2'truncated) specifically ligates the pre-adenylated 5' end of DNA or RNA to the 3 end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2'truncated is expressed from a plasmid in E. coli which encodes the first 2'9 amino acids of the full length T4 RNA Ligase 2' Unlike the full length ligase, T4 Rnl2'truncated is unable to adenylate the 5' end of the substrate, and as a result it cannot ligate the phosphorylated 5' end of RNA or DNA to the 3' end of RNA . This enzyme, also known as Rnl2'(1-2'9) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers).
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