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Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 1 U/mul

Catalog No. 502907689
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The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5primendash3prime DNA polymerase that lacks 3primendash5prime exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 C) and temperatures around 75 C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates and can be used to label DNA-fragments either with radionucleotides digoxigenin fluorescein or biotin.The high processivity absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing especially where the reSOLUTIONon of secondary structures plays a major role.

Catalog No. 50-290-7689 Supplier Sigma Aldrich Fine Chemicals Biosciences Supplier No. 11647687001
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The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5primendash3prime DNA polymerase that lacks 3primendash5prime exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 C) and temperatures around 75 C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates and can be used to label DNA-fragments either with radionucleotides digoxigenin fluorescein or biotin.The high processivity absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing especially where the reSOLUTIONon of secondary structures plays a major role.

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