Applied Biosystems™ TaqMan™ Array Human Transcription of mRNA

Catalog No.	Quantity
44-141-94	1 standard plate

Catalog No. 44-141-94 Supplier Applied Biosystems™ Supplier No. 4414194

Description

Contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes

• The Applied Biosystems™ TaqMan™ Array Human Transcription of mRNA 96-well Plate contains:
  • 44 assays to transcription of mRNA associated genes
  • 4 assays to candidate endogenous control genes
• All assays are plated in duplicate
• Convenient; dried PCR primer and TaqMan™ probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan™ Assay Quality, New Format

Just Add Master Mix and Sample, and Begin Cycling

• Ideal for projects such as validation of microarray and RNAi data, and pathway studies
• The plates include just the right amount of TaqMan™ Assay in each well: enough for one 20 μl reaction
• You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased assays
• Save a significant amount of time setting up your experiments

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering

• Each pack of TaqMan™ Array Plates is shipped with a CD containing the plate configuration map, along with the details of each TaqMan™ Assay
• This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering

Simple to Use

• The plates can be stored at room temperature or at 4°C
• When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument

Compatibility

• TaqMan™ Array Plates have been optimized for use with TaqMan™ Gene Expression Master Mix, but are also compatible with TaqMan™ Universal PCR Master Mix
• For studies using cultured cells, TaqMan™ Array Plates are also compatible (and recommended) with the TaqMan™ Gene Expression Cells-to-Ct™ Kit
• TaqMan™ Array Plates must be run on a real-time PCR instrument with 96-well plate capability
• The plates have been validated for use on Applied Biosystems™ 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 μl reaction volumes

Specifications

• Type: Array Plate
• Format: 96-array Plate
• Species: Human
• Content And Storage: Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 20 μL reaction. The plates can be stored at room temperature or at 4°C.
• Form: Dried Down
• Includes: TaqMan Assay for One 20 uL Reaction
• Internal Probe Modification: MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
• Label or Dye: FAM
• Product Line: TaqMan
• Purification Method: Standard
• Quantity: 1 standard plate
• Reaction Speed: Standard
• Research Category: Gene Regulation

Frequently Asked Questions (FAQs)

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.

Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch

How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.

For Research Use Only. Not for use in diagnostic procedures.