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Invitrogen™ TaqMan™ Cells-to-CT™ Express 1-Step Kit
Description
Skip extraction entirely. Go from cell lysate to results in 1 step.
The TaqMan™ Cells-to-CT™ Express 1-Step Kit enables gene expression analysis directly from cultured cell lysates in a single RT-qPCR reaction. By eliminating RNA purification and combining reverse transcription and quantitative PCR into one streamlined workflow, researchers can generate high-quality gene expression data with fewer processing steps and less hands-on time. The workflow is well suited for early-stage research applications, including target identification, target validation, pathway analysis, and high-throughput screening.
Designed for speed and agility, the workflow combines rapid 5-minute cell lysis with integrated 1-step RT-qPCR, allowing researchers to move from cells to gene expression results in approximately 70 minutes.
Simplify RT-qPCR Workflows for Early Target Validation Research
RT-qPCR remains one of the most widely used methods for measuring gene expression during early-stage drug discovery and biological research. However, traditional workflows typically require RNA purification before amplification, adding time, consumables, and multiple handling steps to the process.
Direct-lysis workflows offer an alternative by enabling cells to be processed and analyzed without RNA extraction. When paired with 1-step RT-qPCR, researchers can move seamlessly from sample preparation to gene expression analysis in a single, continuous workflow.
The TaqMan™ Cells-to-CT™ Express 1-Step Kit was developed to support laboratories performing gene expression-based screening and target validation studies, helping reduce workflow complexity while maintaining the sensitivity, reproducibility, and performance required for confident decision-making.
Key Features & Benefits
- Simplified Gene Expression Workflow – Go directly from cultured mammalian cells to RT-qPCR with no RNA purification required and no separate reverse transcription reaction required. Rapid 5-minute cell lysis with no stop solution or heat inactivation step further streamlines sample preparation.
- Reduced Sample Handling – Minimize pipetting and sample transfer steps to reduce opportunities for user error, contamination, and sample loss.
- Lower Plastic and Consumable Usage – Reduce the number of tubes, plates, pipette tips, and reagents required compared to traditional extraction-based workflows.
- Reliable Gene Expression Results – Generate sensitive and reproducible gene expression data directly from cultured cell lysates using trusted TaqMan™ Gene Expression Assays.
- Scalable for High-Throughput Workflows – Compatible with both manual and automated laboratory environments, featuring an automation-ready workflow that enables efficient processing of large numbers of samples.
- Designed for Early Discovery Research – Enable gene expression analysis for target identification, target validation, and screening applications.
Applications
The TaqMan™ Cells-to-CT™Express 1-Step Kit is designed for RT-qPCR-based gene expression workflows in research and early drug discovery screening labs.
Applications include:
- RT-qPCR gene expression analysis
- Target identification and gene expression-based target validation
- Early-stage drug discovery screening
- High-throughput compound screening
- Pathway activation and response studies
- RNA analysis from cultured mammalian cells
- Candidate target prioritization
- Biomarker and gene expression profiling
- RNA-mediated gene regulation studies
Specifications
Specifications
| Format | Kit |
| Isolation Technology | Direct Cell Lysis |
| Sample Type | Mammalian cells |
| Product Type | Direct Cell Lysis RT-qPCR |
| Quantity | 400 Reactions |
For Research Use Only.
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