Invitrogen™ Human PARC/CCL18 ELISA Kit

Catalog No.	Quantity
EHCCL18X10	10 x 96 Tests

Catalog No. EHCCL18X10 Supplier Invitrogen™ Supplier No. EHCCL18X10

Description

ELISA

The Human PARC/CCL18 ELISA quantitates Hu PARC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PARC. Principle of the method The Human PARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage Inflammatory Protein-4 (MIP-4), also called CCL18, is a chemokine expressed in the lymph nodes, lungs, placenta and bone marrow. Although the MIP-4s receptor remains undetermined, MIP-4 is known to act as a chemoattractant for activated and non-activated T cells.

Specifications

• Accession Number: P55774
• Assay Range: 2-2000 pg/mL
• Assay Sensitivity: 2 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Alternative macrophage activation-associated CC chemokine 1; AMAC1; AMAC-1; CC chemokine ligand 18; CC chemokine PARC; C-C motif chemokine 18; C-C motif chemokine ligand 18; CCL18; CCL18(1-68); CCL18(3-69); CCL18(4-69); chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); chemokine (C-C), dendritic; CKb7; CUL9; CUL-9; cullin 9; cullin-9; DCCK1; DC-CK1; dendritic cell chemokine 1; H7AP1; H-MIP-4; KIAA0708; Macrophage inflammatory protein 4; MIP4; MIP-4; p53-associated parkin-like cytoplasmic protein; PARC; parkin-like cytoplasmic p53 binding protein; pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; small-inducible cytokine A18; UbcH7-associated protein 1Alternative macrophage activation-associated CC chemokine 1; AMAC1; AMAC-1; CC chemokine ligand 18; CC chemokine PARC; C-C motif chemokine 18; C-C motif chemokine ligand 18; CCL18; CCL18(1-68); CCL18(3-69); CCL18(4-69); chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); chemokine (C-C), dendritic; CKb7; CUL9; CUL-9; cullin 9; cullin-9; DCCK1; DC-CK1; dendritic cell chemokine 1; H7AP1; H-MIP-4; KIAA0708; Macrophage inflammatory protein 4; MIP4; MIP-4; p53-associated parkin-like cytoplasmic protein; PARC; parkin-like cytoplasmic p53 binding protein; pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; small-inducible cytokine A18; UbcH7-associated protein 1; AMAC-1; AMAC1; CKb7; DC-CK1; DCCK1; MIP-4; PARC; SCYA18
• Gene ID (Entrez): 6362
• Gene Symbol: CCL18
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: PARC
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min