Invitrogen™ Human TARC/CCL17 ELISA Kit

Catalog No.	Quantity
EHCCL17	96 Tests
EHCCL17X10	10 x 96 Tests
EHCCL17X5	5 x 96 Tests

Catalog No. EHCCL17 Supplier Invitrogen™ Supplier No. EHCCL17

Description

ELISA

The Human TARC/CCL17 ELISA quantitates Hu TARC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TARC. Principle of the method The Human TARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.

Specifications

• Accession Number: Q92583
• Assay Range: 5-2500 pg/mL
• Assay Sensitivity: 5 pg/mL
• Calibration Range: 5 pg/mL to 2500 pg/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: A-152E5.3; ABCD-2; CC chemokine TARC; C-C motif chemokine; C-C motif chemokine 17; C-C motif chemokine ligand 17; CCL17; chemokine (C-C motif) ligand 17; SCYA17; Scya17l; small inducible cytokine subfamily A (Cys-Cys), member 17; small inducible cytokine subfamily A17; small-inducible cytokine A17; T cell-directed CC chemokine; TARC; Thymus and activation-regulated chemokineA-152E5.3; ABCD-2; CC chemokine TARC; C-C motif chemokine; C-C motif chemokine 17; C-C motif chemokine ligand 17; CCL17; chemokine (C-C motif) ligand 17; SCYA17; Scya17l; small inducible cytokine subfamily A (Cys-Cys), member 17; small inducible cytokine subfamily A17; small-inducible cytokine A17; T cell-directed CC chemokine; TARC; Thymus and activation-regulated chemokine; A-152E5.3; ABCD-2; SCYA17; TARC
• Gene ID (Entrez): 6361
• Gene Symbol: CCL17
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: A-152E5.3, ABCD-2, SCYA17, TARC
• Target: TARC
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min