Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, can be used for detection of phosphorylated targets, has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection.Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including: Quantitative Western Blot, Chemiluminescent Western Blots, In-Cell Western™ Assay, In-Gel Western, Protein Array, Glycoprotein Detection